Choice of buffer and use of surface blocking in an enzyme-linked immunosorbent assay for antibodies to Ureaplasma urealyticum serotypes
نویسندگان
چکیده
منابع مشابه
Development of an enzyme-linked immunosorbent assay for serotyping ureaplasma urealyticum strains using monoclonal antibodies.
Ureaplasma urealyticum comprises 14 serotypes. The existing serotyping methods all use polyclonal antibodies. These methods are time-consuming and labor-intensive, and they cannot always be performed on primary isolates; in addition, the results are difficult to interpret. We developed a new enzyme-linked immunosorbent assay (ELISA) method using serotype-specific monoclonal antibodies (MAbs) to...
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متن کاملSerological characterisation of Ureaplasma urealyticum strains by enzyme-linked immunosorbent assay (ELISA).
A modification of enzyme-linked immunosorbent assay (ELISA) was developed for the serological characterisation and identification of strains of Ureaplasma urealyticum. The eight recognised human serotypes of U urealyticum and antisera produced against them were used as reference for the evaluation and standardisation of the method. The serological profile illustrating reactions of antigen with ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1985
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.22.2.323-324.1985